Session Nano4Health: Focus on Technologies for Diagnostic purposes
December 2, 11:15-13:00
Roll-to-roll manufacturing of Lab-on-a-Foil Chips for IVD applications
Jan Hesse PhD, Joanneum Institute
Roll-to-roll UV nanoimprint lithography (R2R-UV-NIL) offers possibilities to manufacture micro- or nanostructures on flexible polymer films with different geometries and configurations in high throughput. In addition, by adjusting the composition of the UV-curable resins used for UV-NIL, various parameters such as surface energy, refractive index and stiffness of the final structure can be tuned. Thus, R2R UV-NIL can address many different application areas, such as drag-reducing structures for aerospace, micro-optical elements in lighting devices, and microfluidic structures for lab-on-chip applications.In the first part of the presentation, the technology of the imprinting line and some applications of UV-NIL structured foils are presented. The second part will focus on the production of microfluidic lab-on-a-foil systems for IVD applications. Our imprinting pilot line enables high-throughput patterning of approx. 4500 biochips/hour on flexible polymer substrates. A second R2R unit is used to print biomolecules such as DNA or proteins onto the pre-structured foils generating sensory elements to the lab-on-a-foil system. After this biofunctionalisation step, a sealing film is laminated onto the fluidic structures, creating closed fluidic channels. Finally, inlet holes and vents are cut into the sealing film with a laser cutter. The lab-on-a-chips produced show similar performance to commercial products, but can be produced with higher throughput and greatly reduced manual handling steps, potentially enabling lower costs in production.
Dr. Jan Hesse studied Physics at the University of Potsdam (Germany) and obtained his PhD in Biophysics at the Johannes Kepler University Linz (Austria) in 2004. In a PostDoc position at Johannes Kepler University Linz he worked on the development of microscopy methods and microarrays for single molecule detection (2004-2006). From 2006-2016 he worked as group leader for fluorescence microscopy and microassays at the Upper Austrian Research GmbH (Austria) and the Center for Advanced Bioanalysis GmbH (Austria). There he focussed on the implementation of DNA detection assays in microfluidic chips and the development of an IVD for the identification of invasive fungal diseases. In 2017 he joined JOANNEUM RESEARCH as head of the research group “Sensors and Functional Printing” where he is working on the development of opto-chemical and biochemical sensors and on manufacturing technologies for Lab-on-Chip systems. Currently his work focusses on R2R-based manufacturing technologies for so-called Lab-on-a-foil systems for in vitro diagnostics. In this context, he is president of the Microfluidics Innovation Hub, an association that bundles the development and manufacturing services of 21 partners and provides these services to customers e.g. in medical diagnostics and food analysis.